Anti-phosphocholine hybridoma antibodies. I. Direct evidence for three distinct families of antibodies in the murine response

J Exp Med. 1981 Feb 1;153(2):352-64. doi: 10.1084/jem.153.2.352.

Abstract

Biochemical and serological studies were performed on more than 400 anti- phosphocholine (PC) hybridoma proteins (HP) derived from six strains of mice; 26 of these HP were examined in detail. All HP possessed specificity for PC, and all those tested contained an H-chain idiotypic determinant, V(H)-PC, which is shared by PC-binding myeloma proteins (BMP) and anti-PC antibodies. Among the HP, three well-defined and distinct families that correlated well with previous studies on serum anti-PC antibodies were identified. The largest group shared idotypic determinants, an L-chain isoelectric focusing (IEF) pattern, and a binding site specificity with the PC-BMP, T15. Using the same criteria, a second group was found to be strikingly similar to another PC-BMP, M603. The third group possessed an idiotypic determinant and an L-chain IEF profile similar to M511, but differences in binding site specificities were observed among the HP. The latter two groups contained members whose L-chain IEF profiles were not identical to other members of that group. Thus, among strains there is a remarkable degree of conservation among responding anti-PC antibodies, in both the kinds of anti-PC families that exist and the immunochemical and structural characteristics of various members within a family. Differences in at least one parameter were observed in each family, demonstrating that even a relatively restricted response is heterogeneous. However, this diversity seems to operate within certain constraints.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies*
  • Binding Sites, Antibody
  • Cell Fusion
  • Choline / analogs & derivatives*
  • Hybrid Cells / immunology*
  • Immunoglobulin Idiotypes
  • Immunoglobulin Light Chains
  • Immunoglobulins / classification
  • Isoelectric Focusing
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • Mice, Inbred CBA
  • Phosphorylcholine / immunology*

Substances

  • Antibodies
  • Immunoglobulin Idiotypes
  • Immunoglobulin Light Chains
  • Immunoglobulins
  • Phosphorylcholine
  • Choline