A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

M L Gefter; D H Margulies; M D Scharff

doi:10.1007/BF01551818

A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

Somatic Cell Genet. 1977 Mar;3(2):231-6. doi: 10.1007/BF01551818.

Authors

M L Gefter, D H Margulies, M D Scharff

PMID: 605383
DOI: 10.1007/BF01551818

Abstract

A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentraion of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Cell Fusion*
Cell Line
Chromosomes
Dose-Response Relationship, Drug
Karyotyping
Multiple Myeloma
Neoplasms, Experimental
Polyethylene Glycols*
Time Factors

Substances

Polyethylene Glycols