Abstract
A cDNA for purpurin, a secreted 20,000 dalton neural retina cell adhesion and survival protein, has been sequenced and expressed in mammalian cells. Purpurin mRNA is found in both embryonic and adult retina, but not the brain, heart, or liver. The protein is highly concentrated in the neural retina between the pigmented epithelium and the outer segments of the photoreceptor cells; it is synthesized by photoreceptor cells. The predicted purpurin sequence contains 196 residues, has approximately 50% sequence homology with serum retinol binding protein, and is a member of the alpha-2 mu-globulin superfamily. Purpurin binds retinol and may play a major role in retinol transport across the interphotoreceptor cell matrix.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Anthraquinones*
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Base Sequence
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Cell Adhesion / drug effects
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Cell Line
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Cell Survival / drug effects
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Chick Embryo
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Cricetinae
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DNA / genetics
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DNA, Recombinant
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Humans
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Lectins / biosynthesis
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Lectins / genetics*
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Molecular Sequence Data
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Peptide Fragments
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Pigment Epithelium of Eye / metabolism
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Retina / cytology
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Retina / embryology
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Retina / metabolism*
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Retinol-Binding Proteins / genetics
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Rod Cell Outer Segment / metabolism
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Sequence Homology, Nucleic Acid
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Transfection
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Vitamin A / metabolism
Substances
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Anthraquinones
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DNA, Recombinant
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Lectins
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Peptide Fragments
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Recombinant Proteins
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Retinol-Binding Proteins
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Vitamin A
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DNA
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purpurin anthraquinone