Abstract
Macrophage-like U-937 cells secrete a 22-kilodalton heparin-binding growth factor that is mitogenic for BALB-3T3 fibroblasts and smooth muscle cells, but not endothelial cells. The amino acid sequence predicted from complementary DNA clones indicates that the mitogen is a new member of the epidermal growth factor (EGF) family. This heparin-binding EGF-like growth factor (HB-EGF) binds to EGF receptors on A-431 epidermoid carcinoma cells and smooth muscle cells, but is a far more potent mitogen for smooth muscle cells than is EGF. HB-EGF is also expressed in cultured human macrophages and may be involved in macrophage-mediated cellular proliferation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cell Division / drug effects
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Cell Line
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Chromatography, Affinity
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Chromatography, High Pressure Liquid
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DNA Replication / drug effects
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Epidermal Growth Factor / genetics
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Epidermal Growth Factor / isolation & purification
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Epidermal Growth Factor / metabolism*
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Epidermal Growth Factor / pharmacology
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ErbB Receptors / metabolism
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Growth Substances / metabolism*
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Heparin / metabolism*
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Humans
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Kinetics
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Macrophages / metabolism*
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Molecular Sequence Data
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Protein Binding
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Sequence Homology, Nucleic Acid
Substances
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Growth Substances
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Epidermal Growth Factor
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Heparin
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ErbB Receptors
Associated data
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GENBANK/M60278
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GENBANK/M61418
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GENBANK/M61419
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GENBANK/M61420
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GENBANK/M61421
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GENBANK/M61422
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GENBANK/M61423
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GENBANK/M61424
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GENBANK/M61425
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GENBANK/M61426