Expression of RAB27B is up-regulated in senescent human cells

Michihiko Fujii; Yoshinori Kawai; Morio Endoh; Mohammad Nazir Hossain; Kazuhiko Nakabayashi; Dai Ayusawa

doi:10.1016/j.mad.2006.03.001

Expression of RAB27B is up-regulated in senescent human cells

Mech Ageing Dev. 2006 Jul;127(7):639-42. doi: 10.1016/j.mad.2006.03.001. Epub 2006 Apr 18.

Authors

Michihiko Fujii¹, Yoshinori Kawai, Morio Endoh, Mohammad Nazir Hossain, Kazuhiko Nakabayashi, Dai Ayusawa

Affiliation

¹ Division of Biochemistry, Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, Yokohama 244-0813, Japan.

PMID: 16620919
DOI: 10.1016/j.mad.2006.03.001

Abstract

Immortal SVts8 cells that express thermolabile SV40 T antigen exhibit a senescence-like phenomenon upon inactivation of the T antigen. By using a cDNA subtractive hybridization technique, RAB27B, a member of the RAB GTPase family, was found to be up-regulated in senescent SVts8 cells. The up-regulation of RAB27B depends on the p53 gene. Enhanced expression was also observed in replicative senescence in normal human fibroblasts.

MeSH terms

Cell Line, Transformed
Cellular Senescence / physiology*
Fibroblasts / cytology
Fibroblasts / physiology*
Gene Expression Profiling
Humans
Tumor Suppressor Protein p53 / metabolism
Up-Regulation / physiology*
rab GTP-Binding Proteins / biosynthesis*
rab GTP-Binding Proteins / genetics
rab27 GTP-Binding Proteins

Substances

Tumor Suppressor Protein p53
rab27 GTP-Binding Proteins
RAB27A protein, human
Rab27B protein, human
rab GTP-Binding Proteins