Abstract
Light-dependent deactivation of rhodopsin as well as homologous desensitization of beta-adrenergic receptors involves receptor phosphorylation that is mediated by the highly specific protein kinases rhodopsin kinase (RK) and beta-adrenergic receptor kinase (beta ARK), respectively. We report here the cloning of a complementary DNA for RK. The deduced amino acid sequence shows a high degree of homology to beta ARK. In a phylogenetic tree constructed by comparing the catalytic domains of several protein kinases, RK and beta ARK are located on a branch close to, but separate from the cyclic nucleotide-dependent protein kinase and protein kinase C subfamilies. From the common structural features we conclude that both RK and beta ARK are members of a newly delineated gene family of guanine nucleotide-binding protein (G protein)-coupled receptor kinases that may function in diverse pathways to regulate the function of such receptors.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cattle
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Cloning, Molecular
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Cyclic AMP-Dependent Protein Kinases*
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Eye Proteins*
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G-Protein-Coupled Receptor Kinase 1
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Gene Expression
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Phylogeny
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Polymerase Chain Reaction
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Protein Kinases / chemistry*
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RNA, Messenger / genetics
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Receptors, Adrenergic, beta / metabolism
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Restriction Mapping
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Rhodopsin / metabolism
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Sequence Alignment
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Transfection
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beta-Adrenergic Receptor Kinases
Substances
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Eye Proteins
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Oligonucleotides
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RNA, Messenger
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Receptors, Adrenergic, beta
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Rhodopsin
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Protein Kinases
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Cyclic AMP-Dependent Protein Kinases
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G-Protein-Coupled Receptor Kinase 1
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beta-Adrenergic Receptor Kinases
Associated data
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GENBANK/M73836
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GENBANK/S57442
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GENBANK/S57444
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GENBANK/S57448
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GENBANK/S57457
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GENBANK/S57504
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GENBANK/S57506
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GENBANK/S57596
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GENBANK/S58152
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GENBANK/X59603