Abstract
MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3' Untranslated Regions
-
Adenosine / metabolism*
-
Animals
-
Embryo, Nonmammalian / physiology*
-
Embryonic Development*
-
Female
-
Gene Expression Profiling
-
Gene Expression Regulation, Developmental*
-
Genes, Reporter
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism
-
MicroRNAs / physiology*
-
Mutation
-
Oligonucleotide Array Sequence Analysis
-
Polymers / metabolism*
-
Protein Biosynthesis
-
RNA Stability
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism*
-
Ribonuclease III / genetics
-
Ribonuclease III / metabolism
-
Transcription, Genetic
-
Up-Regulation
-
Zebrafish / embryology
-
Zygote / physiology
Substances
-
3' Untranslated Regions
-
MicroRNAs
-
Polymers
-
RNA, Messenger
-
Green Fluorescent Proteins
-
polyadenosine
-
Ribonuclease III
-
Adenosine