Transcriptome analysis of the murine forelimb and hindlimb autopod

Dev Dyn. 2005 Sep;234(1):74-89. doi: 10.1002/dvdy.20514.

Abstract

To gain insight into the coordination of gene expression profiles during forelimb and hindlimb differentiation, a transcriptome analysis of mouse embryonic autopod tissues was performed using Affymetrix Murine Gene Chips (MOE-430). Forty-four transcripts with expression differences higher than 2-fold (T test, P < or = 0.05) were detected between forelimb and hindlimb tissues including 38 new transcripts such as Rdh10, Frzb, Tbx18, and Hip that exhibit differential limb expression. A comparison of gene expression profiles in the forelimb, hindlimb, and brain revealed 24 limb-signature genes whose expression was significantly enriched in limb autopod versus brain tissue (fold change >2, P < or = 0.05). Interestingly, the genes exhibiting enrichment in the developing autopod also segregated into significant fore- and hindlimb-specific clusters (P < or = 0.05) suggesting that by E 12.5, unique gene combinations are being used during the differentiation of each autopod type.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / embryology
  • Brain / metabolism
  • Cell Differentiation
  • Forelimb / cytology
  • Forelimb / embryology*
  • Forelimb / metabolism
  • Gene Expression Profiling
  • Hindlimb / cytology
  • Hindlimb / embryology*
  • Hindlimb / metabolism
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • RNA / metabolism*
  • Time Factors
  • Tretinoin / metabolism

Substances

  • Tretinoin
  • RNA