Transforming growth factor-beta1 induces LMO7 while enhancing the invasiveness of rat ascites hepatoma cells

Cancer Lett. 2005 Mar 18;220(1):95-9. doi: 10.1016/j.canlet.2004.07.023.

Abstract

We have previously shown that transforming growth factor-beta1 (TGF-beta1) markedly stimulates the invasive capacity of rat ascites hepatoma AH130 W1 cells in vitro and in vivo. A differential hybridization procedure was used to isolate genes that were specifically up-regulated in TGF-beta1 treated W1 cells. Among ten independent cDNA clones, we focused on LMO7 and a variant isoform, LMO7S, that was generated by alternative splicing. LMO7 had PDZ and LIM domains, while LMO7S had only PDZ domain. TGF-beta1 up-regulated expression levels of LMO7 and LMO7S. LMO7 expression was up-regulated in the highly metastatic clone MM1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascites / metabolism
  • Carcinoma, Hepatocellular / metabolism*
  • Homeodomain Proteins / biosynthesis*
  • Liver Neoplasms / metabolism*
  • Neoplasm Invasiveness
  • Rats
  • Transcription Factors / biosynthesis*
  • Transforming Growth Factor beta / pharmacology*
  • Transforming Growth Factor beta1
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • Homeodomain Proteins
  • LMO7 protein, rat
  • Tgfb1 protein, rat
  • Transcription Factors
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1