Abstract
Wild-type mouse brain endothelial (bEND) cells transformed with the polyoma virus middle-T proliferate rapidly in culture and form hemangiomas in mice. These cells express high levels of platelet/endothelial cell adhesion molecule-1 (PECAM-1), a molecule shown to be important during hemangioma formation. In this study, we have examined the ability of polyoma virus middle-T-transformed mouse bEND cells prepared from PECAM-1-/- mice to proliferate in culture and form hemangiomas in mice. We show that these cells express a number of endothelial cell markers and share a similar morphology with PECAM-1+/+ bEND cells. PECAM-1-/- bEND cells exhibit a limited ability to form tubes in Matrigel and rapidly form hemangioma when injected into nude mice, very similar to PECAM-1+/+ bEND cells. These cells, however, have increased proliferation, slower migration, altered endothelial cell adhesion molecule expression, and are less adherent when compared to PECAM-1+/+ bEND cells. Therefore, lack of PECAM-1 expression impacts polyoma middle-T-transformed endothelial cell proliferative, adhesive, and migratory properties without impacting their ability to rapidly form hemangiomas in mice or poorly organize to capillary-like structures in Matrigel.
2005 Wiley-Liss, Inc.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, CD
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Antigens, Viral, Tumor / genetics
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Antigens, Viral, Tumor / metabolism*
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Biomarkers
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Cadherins / metabolism
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Cell Adhesion / genetics
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Cell Line, Transformed
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Cell Movement / genetics
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Cell Proliferation
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Neoplastic / metabolism
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Cell Transformation, Viral / genetics*
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Collagen
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Drug Combinations
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Endothelial Cells / metabolism*
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Endothelial Cells / virology
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Extracellular Matrix Proteins / metabolism
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Hemangioma / genetics
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Hemangioma / metabolism*
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Hemangioma / virology
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Laminin
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Nude
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Myosin Heavy Chains
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Neoplasm Transplantation
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Neovascularization, Pathologic / genetics
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Nonmuscle Myosin Type IIB
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Platelet Endothelial Cell Adhesion Molecule-1 / genetics*
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Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
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Polyomavirus / metabolism*
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Proteoglycans
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Vascular Cell Adhesion Molecule-1 / metabolism
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Vascular Endothelial Growth Factor Receptor-2 / metabolism
Substances
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Antigens, CD
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Antigens, Viral, Tumor
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Biomarkers
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Cadherins
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Drug Combinations
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Extracellular Matrix Proteins
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Laminin
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Platelet Endothelial Cell Adhesion Molecule-1
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Proteoglycans
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Vascular Cell Adhesion Molecule-1
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cadherin 5
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matrigel
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Collagen
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Vascular Endothelial Growth Factor Receptor-2
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Nonmuscle Myosin Type IIB
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nonmuscle myosin type IIB heavy chain
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Myosin Heavy Chains