Gene expression profiling of purified rat retinal ganglion cells

Invest Ophthalmol Vis Sci. 2004 Aug;45(8):2503-13. doi: 10.1167/iovs.03-1391.

Abstract

Purpose: The phenotype of specialized cells arises, in part, from their characteristic gene expression patterns. Retinal ganglion cells (RGCs) are of wide interest in neuroscience and die in glaucoma and other optic neuropathies. In this study the genes expressed by RGCs were profiled by expressed sequence tag (EST) analysis.

Methods: ESTs were generated from a cDNA library constructed from RGCs isolated by immunopanning. The RGC genes were compared with published microarray expression profiles from 13 different neural regions. Immunohistochemistry was performed by standard methods.

Results: Clustering of 4791 RGC ESTs identified 2360 unique gene clusters. Of these, 60% represented known genes, 27% uncharacterized genes/ESTs, and 13% novel sequence. Unexpectedly, one of the largest RGC clusters, RESP18, corresponded to a neuroendocrine-specific gene preferentially expressed in the hypothalamus. RESP18 immunoreactivity within the retina was found mainly in the RGC layer. DDAH1, a gene involved in nitric oxide metabolism, was localized to RGC and amacrine layers. Comparison of gene expression patterns across neuronal regions revealed a prominent subset of RGC genes that were overexpressed in dorsal root and trigeminal ganglia. To narrow the search for candidate disease-related genes, RGC genes were mapped to known disease loci for optic neuropathies.

Conclusions: This work is one of the first efforts to profile gene expression in a purified population of retinal neurons, the RGCs. The profiling, in addition to revealing both known and novel genes underlying the RGC phenotype, also uncovered common patterns of gene expression between RGCs and other sensory ganglia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Expressed Sequence Tags
  • Eye Proteins / genetics
  • Gene Expression / physiology*
  • Gene Expression Profiling*
  • Gene Library
  • Immunoenzyme Techniques
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Optic Nerve Diseases / genetics
  • Phenotype
  • Rats
  • Rats, Sprague-Dawley
  • Retinal Ganglion Cells / cytology
  • Retinal Ganglion Cells / metabolism*
  • Sequence Tagged Sites

Substances

  • Eye Proteins
  • Nerve Tissue Proteins
  • regulated endocrine secretory protein 18