Angiogenesis-related factors derived from retinal glial (Müller) cells in hypoxia

Neuroreport. 2004 Jul 19;15(10):1633-7. doi: 10.1097/01.wnr.0000133071.00786.a4.

Abstract

Retinal glial (Müller) cells may play a major role in vascular eye diseases as they secrete vascular endothelial growth factor (VEGF), a hypoxia-induced angiogenic cytokine. They also release significant amounts of the anti-angiogenic factors, transforming growth factor (TGF)-beta2, pigment epithelium derived factor (PEDF), and thrombospondin-1 (TSP-1). Exposure of human (MIO-M1) and guinea-pig Müller cells to hypoxia resulted in a decreased release of TGF-beta2 and PEDF but in an elevated secretion of TSP-1. When retinal endothelial cells were exposed to VEGF/anti-angiogenic factor ratios mimicking those found in culture media of Müller cells under normoxia or hypoxia, their proliferation was significantly inhibited by TGF-beta2, PEDF or TSP-1. Thus Müller cells may provide a permanent anti-proliferative condition for retinal endothelial cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Animals, Newborn
  • Cell Division / physiology
  • Cell Hypoxia
  • Cells, Cultured
  • Cytokines / metabolism
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Endothelial Cells / metabolism
  • Enzyme-Linked Immunosorbent Assay / methods
  • Eye Proteins*
  • Guinea Pigs
  • Humans
  • Nerve Growth Factors*
  • Neuroglia / metabolism*
  • Proteins / metabolism*
  • Proteins / pharmacology
  • Retina / cytology*
  • Serpins / metabolism*
  • Serpins / pharmacology
  • Thrombospondin 1 / metabolism*
  • Thrombospondin 1 / pharmacology
  • Transforming Growth Factor beta / metabolism*
  • Transforming Growth Factor beta / pharmacology
  • Transforming Growth Factor beta2
  • Vascular Endothelial Growth Factor A / metabolism*
  • Vascular Endothelial Growth Factor A / pharmacology

Substances

  • Cytokines
  • Eye Proteins
  • Nerve Growth Factors
  • Proteins
  • Serpins
  • TGFB2 protein, human
  • Thrombospondin 1
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta2
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • pigment epithelium-derived factor