Abstract
Transcription of the platelet-derived growth factor (PDGF) A-chain gene is activated in cells exposed to growth factors. We now have identified a homopurine/homopyrimidine domain in the promoter region of the PDGF A-chain gene that exhibits S1 nuclease sensitivity in vitro and that contains a novel binding site (5'-TCCTCCTCCTCCTC-3') for the growth factor inducible transcription factor EGR-1 as demonstrated in gel mobility shift assays. Sequences similar to this novel EGR-1 binding site were observed also in five growth-related genes and shown to bind to EGR-1 in competition assays, suggesting that EGR-1 may influence the transcriptional regulation of these growth-related genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / metabolism*
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Growth Substances / pharmacology
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Macromolecular Substances
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Platelet-Derived Growth Factor / genetics*
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Promoter Regions, Genetic*
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Sequence Homology, Nucleic Acid
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Single-Strand Specific DNA and RNA Endonucleases / metabolism*
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Substrate Specificity
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Transcription Factors / biosynthesis
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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Growth Substances
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Macromolecular Substances
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Oligodeoxyribonucleotides
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Platelet-Derived Growth Factor
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Transcription Factors
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Single-Strand Specific DNA and RNA Endonucleases