Endothelial leukocyte adhesion molecule-1 in endotoxin-induced uveitis

Invest Ophthalmol Vis Sci. 1992 Aug;33(9):2626-30.

Abstract

Expression of endothelial leukocyte adhesion molecule-1 (ELAM-1) on endothelial cells leads to the attachment of polymorphonuclear leukocytes. The sequential expression of ELAM-1 and major histocompatibility complex (MHC) class II antigen was examined in the eyes of 59 Lewis rats with endotoxin-induced uveitis (EIU) after the injection of Salmonella typhimurium endotoxin. The eyes were enucleated at 2-hr intervals. Hematoxylin and eosin-stained paraffin-embedded sections and immunohistochemically stained cryostat sections were graded by two masked observers. The MHC class II antigen was expressed on cells in the iris and ciliary body 4 hr after injection of endotoxin and on the corneal endothelium, 8 hr postinjection. It was found that ELAM-1 was expressed first on cells of the ciliary body and iris 10 hr after the injection of endotoxin and on the corneal endothelium, 22 hr postinjection. Clinical and histopathologic disease developed 16 hr postinjection. Adherence of polymorphonuclear cells to the corneal endothelium was observed at the time of ELAM-1 expression. In conclusion, expression of ELAM-1 on ocular tissue occurred in EIU and appeared to promote polymorphonuclear cell accumulation in the anterior segment of the eye.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anterior Eye Segment / metabolism
  • Antibodies, Monoclonal
  • Bacterial Toxins
  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism*
  • E-Selectin
  • Endothelium, Corneal / immunology
  • Endothelium, Corneal / metabolism
  • Endotoxins
  • Enterotoxins
  • Female
  • Histocompatibility Antigens Class II / metabolism
  • Immunoenzyme Techniques
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / metabolism*
  • Neutrophils / metabolism
  • Rats
  • Rats, Inbred Lew
  • Receptors, Immunologic / metabolism
  • Salmonella
  • Uveitis, Anterior / chemically induced
  • Uveitis, Anterior / immunology
  • Uveitis, Anterior / metabolism*

Substances

  • Antibodies, Monoclonal
  • Bacterial Toxins
  • Cell Adhesion Molecules
  • E-Selectin
  • Endotoxins
  • Enterotoxins
  • Histocompatibility Antigens Class II
  • Membrane Glycoproteins
  • Receptors, Immunologic
  • salmonella toxin