N-Myc downstream-regulated gene 2 (NDRG2) inhibits glioblastoma cell proliferation

Int J Cancer. 2003 Sep 1;106(3):342-7. doi: 10.1002/ijc.11228.

Abstract

The most severe form of brain glioma, glioblastoma (GBM), is highly malignant and usually resistant to chemotherapy. Therefore, discovery of new targets for gene therapy is important. Using subtraction cloning, we identified the human N-Myc downstream-regulated gene 2 (hNDRG2), located at chromosome 14q11.2, as a gene that is significantly suppressed in GBM tissues. Semiquantitative RT-PCR showed that the hNDRG2 gene transcript is expressed in normal brain tissue and low-grade gliomas but is present at low levels in 15 of 27 (56%) human GBM tissues and all of the 6 human glioblastoma cell lines examined. Furthermore, transfection of human glioblastoma U373 and U138 cells with a cDNA encoding hNDRG2 markedly reduced the cell proliferation. Our findings provide the first evidence to suggest that hNDRG2 may play a role in glioblastoma carcinogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism
  • Blotting, Northern
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology*
  • Cell Division
  • Chromosomes, Human, Pair 14 / genetics
  • Chromosomes, Human, Pair 14 / metabolism
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • DNA, Complementary / metabolism
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic*
  • Glioblastoma / genetics
  • Glioblastoma / metabolism
  • Glioblastoma / pathology*
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism
  • Proteins / genetics*
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution
  • Transfection
  • Tumor Cells, Cultured / metabolism
  • Tumor Cells, Cultured / pathology
  • Tumor Suppressor Proteins

Substances

  • Biomarkers, Tumor
  • DNA, Complementary
  • NDRG2 protein, human
  • Neoplasm Proteins
  • Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Tumor Suppressor Proteins

Associated data

  • GENBANK/AF159092