Abstract
The mitogen-activated protein (MAP) kinase pathways has been shown to be necessary for mitogen-stimulated proliferation, but its role in cell migration has not been fully understood. In this study, we investigated the possible contribution of signaling pathways through c-Jun in platelet-derived growth factor (PDGF)-BB directed cell migration in rat aortic vascular smooth muscle cells (VSMCs) infected with a recombinant adenovirus containing the dominant-negative c-Jun (Ad-DN-c-Jun). DN-c-Jun protein was expressed dose-dependently in VSMCs infected with Ad-DN-c-Jun. Expression of DN-c-Jun significantly inhibited VSMC migration induced by PDGF-BB. Our results provide the first evidence that signaling pathways through c-Jun participates in cell migration induced by PDGF-BB in addition to other MAP kinase pathways in VSMCs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aorta, Thoracic / cytology
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Aorta, Thoracic / drug effects
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Cell Movement / drug effects*
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Enzyme Inhibitors / pharmacology
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Flavonoids / pharmacology
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Genes, Dominant
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Imidazoles / pharmacology
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In Vitro Techniques
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JNK Mitogen-Activated Protein Kinases*
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MAP Kinase Kinase 4
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Male
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Mitogen-Activated Protein Kinase Kinases / physiology
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Mitogen-Activated Protein Kinases / antagonists & inhibitors
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Muscle, Smooth, Vascular / cytology*
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Muscle, Smooth, Vascular / drug effects
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Platelet-Derived Growth Factor / antagonists & inhibitors*
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Platelet-Derived Growth Factor / pharmacology*
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Proto-Oncogene Proteins c-jun / pharmacology*
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Pyridines / pharmacology
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Rats
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Rats, Sprague-Dawley
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Signal Transduction / drug effects
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Signal Transduction / physiology
Substances
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Enzyme Inhibitors
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Flavonoids
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Imidazoles
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Platelet-Derived Growth Factor
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Proto-Oncogene Proteins c-jun
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Pyridines
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases
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MAP Kinase Kinase 4
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Mitogen-Activated Protein Kinase Kinases
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SB 203580
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one