Functional and molecular characterisation of mammary side population cells

Breast Cancer Res. 2003;5(1):R1-8. doi: 10.1186/bcr547. Epub 2002 Oct 14.

Abstract

Background: Breast cancer is thought to arise in mammary epithelial stem cells. However, the identity of these stem cells is unknown.

Methods: Studies in the haematopoetic and muscle systems show that stem cells have the ability to efflux the dye Hoechst 33342. Cells with this phenotype are referred to as the side population (SP). We have adapted the techniques from the haematopoetic and muscle systems to look for a mammary epithelial SP.

Results: Of mammary epithelial cells isolated from both the human and mouse mammary epithelia, 0.2-0.45% formed a distinct SP. The SP was relatively undifferentiated but grew as typical differentiated epithelial clones when cultured. Transplantation of murine SP cells at limiting dilution into cleared mammary fat pads generated epithelial ductal and lobuloalveolar structures.

Conclusion: These data demonstrate the existence of an undifferentiated SP in human and murine mammary epithelium. Purified SP cells are a live single-cell population that retain the ability to differentiate in vitro and in vivo. Studies of haematopoetic cells have suggested that the SP phenotype constitutes a universal stem cell marker. This work therefore has implications for mammary stem cell biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters / genetics
  • Adipose Tissue / cytology*
  • Animals
  • Benzimidazoles / metabolism
  • Breast / cytology*
  • Cell Differentiation
  • Cell Division / drug effects
  • Cell Transplantation
  • Cells, Cultured
  • Clone Cells / cytology
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Female
  • Flow Cytometry
  • Humans
  • Keratin-14
  • Keratins / biosynthesis
  • Mammary Glands, Animal / cytology*
  • Mice
  • Neoplasm Proteins*
  • RNA / genetics
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Verapamil / pharmacology

Substances

  • ABCG2 protein, human
  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters
  • Benzimidazoles
  • KRT14 protein, human
  • Keratin-14
  • Krt14 protein, mouse
  • Neoplasm Proteins
  • RNA
  • Keratins
  • Verapamil
  • bisbenzimide ethoxide trihydrochloride