Correlation of regenerable opsin with rod ERG signal in Rpe65-/- mice during development and aging

Invest Ophthalmol Vis Sci. 2003 Jan;44(1):310-5. doi: 10.1167/iovs.02-0567.

Abstract

Purpose: RPE65 has been shown to be essential for the production of 11-cis retinal by the retinal pigment epithelium. Mutations in RPE65 are known to be associated with severe forms of early-onset retinal dystrophy. This project was designed to determine the amount of regenerable opsin in Rpe65-/- mice during development and aging, and to examine the function of this rhodopsin by electroretinography (ERG).

Methods: Young and aged Rpe65-/- and wild-type (WT) mice were dark adapted. Endogenous rhodopsin and regenerable opsin were measured using absorption-difference spectrophotometry. Photoreceptor function was assessed with scotopic single-flash ERGs and photoreceptors were counted in histologic sections. Opsin's primary structure was analyzed by mass-spectrometric mapping.

Results: Unlike WT mice, amounts of regenerable opsin in Rpe65-/- mice decreased significantly with age, which correlated with a decrease in the number of photoreceptors and a decline in ERG amplitudes. Opsin structure, however, did not change. No endogenous levels of rhodopsin were measurable in the Rpe65-/- mice (detection limit: 0.225 pmol). 11-cis Retinal injections resulted in the regeneration of similar amounts of rhodopsin and improved rod function in a comparable way, irrespective of age.

Conclusions: In the aged Rpe65-/- mouse, opsin levels decrease because of the loss of photoreceptors. The remaining opsin is structurally intact, and the components of the phototransduction cascade and the retinal circuitry remain functional, despite the absence of normal photoreceptor activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / physiology*
  • Animals
  • Carrier Proteins
  • Dark Adaptation
  • Electroretinography
  • Eye Proteins / physiology*
  • Injections
  • Mass Spectrometry
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microspectrophotometry
  • Proteins / physiology*
  • Regeneration
  • Retinal Rod Photoreceptor Cells / growth & development*
  • Retinal Rod Photoreceptor Cells / physiology*
  • Retinaldehyde / pharmacology
  • Rod Opsins / biosynthesis*
  • Vision, Ocular / physiology
  • cis-trans-Isomerases

Substances

  • Carrier Proteins
  • Eye Proteins
  • Proteins
  • Rod Opsins
  • retinoid isomerohydrolase
  • cis-trans-Isomerases
  • Retinaldehyde