Abstract
Neovascularization characterizes diabetic retinopathy and choroidal neovascularization associated with age-related macular degeneration, the most common causes of severe visual loss in the developed world. Gene transfer to the eye using adeno-associated viral (AAV) vectors is a promising new treatment for inherited and acquired ocular diseases. We used an AAV vector with rapid onset and high levels of gene expression in the retina to deliver three anti-angiogenic factors (pigment epithelium-derived factor, tissue inhibitor of metalloproteinase-3, and endostatin) to the eyes of mice in a mouse model of retinopathy of prematurity. All three vectors inhibited ischemia-induced neovascularization.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Angiogenesis Inhibitors / genetics
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Angiogenesis Inhibitors / pharmacology*
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Collagen / genetics
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Collagen / pharmacology*
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Cytomegalovirus / genetics
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Dependovirus / genetics
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Endostatins
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Endothelium, Vascular / metabolism
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Eye Proteins*
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Gene Transfer Techniques*
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Genes, Reporter
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Genetic Vectors
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Humans
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In Vitro Techniques
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Nerve Growth Factors*
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Peptide Fragments / genetics
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Peptide Fragments / pharmacology*
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Promoter Regions, Genetic
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Proteins / genetics
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Retinal Neovascularization / drug therapy*
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Serpins / genetics
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Tissue Inhibitor of Metalloproteinase-3
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Tissue Inhibitor of Metalloproteinases / genetics
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Transduction, Genetic
Substances
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Angiogenesis Inhibitors
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Endostatins
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Eye Proteins
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Nerve Growth Factors
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Peptide Fragments
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Proteins
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Serpins
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TIMP3 protein, human
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Tissue Inhibitor of Metalloproteinase-3
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Tissue Inhibitor of Metalloproteinases
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pigment epithelium-derived factor
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Collagen