Monocyte chemotactic protein-1 secretion and expression after Toxoplasma gondii infection in vitro depend on the stage of the parasite

FEMS Microbiol Lett. 2002 Aug 27;214(1):45-9. doi: 10.1111/j.1574-6968.2002.tb11323.x.

Abstract

Infection of human fibroblasts with tachyzoites of RH and Prugniaud strains, two different strains of Toxoplasma gondii, significantly increased monocyte chemotactic protein (MCP)-1 secretion contrary to what happened with bradyzoites of the cystogenetic strain. Quantification of MCP-1 mRNA by RT-PCR showed that this phenomenon is regulated at the transcriptional level. Thus, the stage of parasite can be deciding in MCP-1 induction since only tachyzoites induced MCP-1 expression and secretion. MCP-1 induced by tachyzoites could be involved in cell recruitment, as shown by the quantification of MCP1 ARNm by real-time PCR (LightCycler, Roche Diagnostics), in the pathogenesis of T. gondii infection.

MeSH terms

  • Animals
  • Cell Line
  • Chemokine CCL2 / genetics
  • Chemokine CCL2 / metabolism*
  • Fibroblasts / parasitology
  • Gene Expression Regulation
  • Humans
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism
  • RNA, Protozoan / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Toxoplasma / growth & development*
  • Toxoplasma / metabolism
  • Toxoplasma / pathogenicity*
  • Transcription, Genetic

Substances

  • Chemokine CCL2
  • RNA, Messenger
  • RNA, Protozoan