Diverse developmental programs of Xenopus laevis metamorphosis are inhibited by a dominant negative thyroid hormone receptor

A M Schreiber; B Das; H Huang; N Marsh-Armstrong; D D Brown

doi:10.1073/pnas.191361698

Diverse developmental programs of Xenopus laevis metamorphosis are inhibited by a dominant negative thyroid hormone receptor

Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10739-44. doi: 10.1073/pnas.191361698. Epub 2001 Aug 21.

Authors

A M Schreiber¹, B Das, H Huang, N Marsh-Armstrong, D D Brown

Affiliation

¹ Carnegie Institution of Washington, Department of Embryology, 115 West University Parkway, Baltimore, MD 21210, USA.

Abstract

Metamorphosis of anuran tadpoles is controlled by thyroid hormone (TH). Here we demonstrate that transgenic Xenopus laevis tadpoles expressing a dominant negative form of TH receptor-alpha are resistant to a wide variety of the metamorphic changes induced by TH. This result confirms that TH receptors mediate both early and late developmental programs of metamorphosis as diverse as growth in the brain, limb buds, nose and Meckel's cartilage, remodeling of the intestine, and death and resorption of the gills and tail.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Animals, Genetically Modified
CHO Cells
Collagen / genetics
Cricetinae
Cytomegalovirus / genetics
Gene Expression
Green Fluorescent Proteins
Luminescent Proteins / genetics
Metamorphosis, Biological*
Promoter Regions, Genetic
Receptors, Thyroid Hormone / genetics
Receptors, Thyroid Hormone / metabolism*
Thyroid Hormones / metabolism
Transgenes
Xenopus laevis / growth & development*

Substances

Luminescent Proteins
Receptors, Thyroid Hormone
Thyroid Hormones
Green Fluorescent Proteins
Collagen