Abstract
We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Antibody-Producing Cells / immunology*
-
Cell Fusion
-
Cell Line
-
Cell Transformation, Neoplastic
-
Enzyme-Linked Immunosorbent Assay
-
Hybrid Cells / immunology*
-
Immunoglobulin Heavy Chains*
-
Immunoglobulin Light Chains*
-
Mice
-
Mice, Inbred C57BL
-
Multiple Myeloma / immunology*
-
Rabbits
Substances
-
Immunoglobulin Heavy Chains
-
Immunoglobulin Light Chains