Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles

C J Roberts; B Nelson; M J Marton; R Stoughton; M R Meyer; H A Bennett; Y D He; H Dai; W L Walker; T R Hughes; M Tyers; C Boone; S H Friend

doi:10.1126/science.287.5454.873

Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles

Science. 2000 Feb 4;287(5454):873-80. doi: 10.1126/science.287.5454.873.

Authors

C J Roberts¹, B Nelson, M J Marton, R Stoughton, M R Meyer, H A Bennett, Y D He, H Dai, W L Walker, T R Hughes, M Tyers, C Boone, S H Friend

Affiliation

¹ Rosetta Inpharmatics, 12040 115th Avenue Northeast, Kirkland, WA 98034, USA.

PMID: 10657304
DOI: 10.1126/science.287.5454.873

Abstract

Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs-Fus3p and Kss1p-revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle Proteins*
Cyclin-Dependent Kinase Inhibitor Proteins
Fungal Proteins / genetics
Fungal Proteins / metabolism
Fungal Proteins / physiology
G1 Phase
Gene Expression Profiling*
Gene Expression Regulation, Fungal*
Genome, Fungal
Lipoproteins / pharmacology
Lipoproteins / physiology
MAP Kinase Signaling System*
Mating Factor
Mitogen-Activated Protein Kinases / metabolism
Multigene Family
Oligonucleotide Array Sequence Analysis
Peptides / pharmacology
Peptides / physiology
Pheromones
Protein Kinase C / metabolism
Repressor Proteins*
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae / physiology
Saccharomyces cerevisiae Proteins*
Transcription Factors / metabolism
Transcriptional Activation

Substances

Cell Cycle Proteins
Cyclin-Dependent Kinase Inhibitor Proteins
FAR1 protein, S cerevisiae
Fungal Proteins
Lipoproteins
MFA2 protein, S cerevisiae
Peptides
Pheromones
Repressor Proteins
STE12 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Mating Factor
Protein Kinase C
FUS3 protein, S cerevisiae
Mitogen-Activated Protein Kinases