Abstract
Posttranscriptional gene silencing (PTGS) is a nucleotide sequence-specific defense mechanism that can target both cellular and viral mRNAs. Here, three types of transgene-induced PTGS and one example of virus-induced PTGS were analyzed in plants. In each case, antisense RNA complementary to the targeted mRNA was detected. These RNA molecules were of a uniform length, estimated at 25 nucleotides, and their accumulation required either transgene sense transcription or RNA virus replication. Thus, the 25-nucleotide antisense RNA is likely synthesized from an RNA template and may represent the specificity determinant of PTGS.
Publication types
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Comment
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Oxidoreductases / genetics
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Gene Expression Regulation, Plant*
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Gene Silencing*
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Green Fluorescent Proteins
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Luminescent Proteins / genetics
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Nicotiana / genetics
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Plants / enzymology
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Plants / genetics
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Plants / virology
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Plants, Genetically Modified
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Plants, Toxic
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Potexvirus / genetics
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RNA, Antisense / analysis
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RNA, Antisense / physiology*
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RNA, Messenger / analysis
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RNA, Messenger / physiology
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RNA, Plant / analysis
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RNA, Plant / physiology*
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RNA, Viral / metabolism
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Transcription, Genetic
Substances
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Luminescent Proteins
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RNA, Antisense
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RNA, Messenger
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RNA, Plant
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RNA, Viral
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Green Fluorescent Proteins
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Amino Acid Oxidoreductases
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1-aminocyclopropane-1-carboxylic acid oxidase