Toxic effects of mitomycin-C on cultured corneal keratocytes and endothelial cells

J Ocul Pharmacol Ther. 1999 Oct;15(5):401-11. doi: 10.1089/jop.1999.15.401.

Abstract

Improper use of mitomycin-C in ocular medication may result in damage to corneal cells. In this study, the toxic effects of mitomycin-C on cultured porcine keratocytes and endothelial cells were estimated by MTT, 3H-thymidine uptake and cellular counting assay methods. It was found that mitomycin-C caused a dose-dependent toxic effect to keratocytes and endothelial cells. Both cells were treated with mitomycin-C at the concentration ranging from 100, 10, 1, 0.1 to 0.01 microg/ml for 3 min, 5 min or 100 min. The 50% inhibitory dose (ID50) of mitomycin-C to keratocytes and endothelial cells as measured by MTT assay was 0.40, 0.18, 0.16 mg/ml and 0.27, 0.15, 0.14 mg/ml, respectively, after 3, 5 and 100 minutes drug treatment. The ID50 for keratocytes and endothelial cells as measured by 3H-thymidine uptake immediately, 1 day and 7 days after 100 minutes mitomycin-C treatment was 0.3, 0.0002, 143.2 microg/ml and 45.1, 101.1, 450.2 microg/ml, respectively. The ID50 for keratocytes and endothelial cells as measured by cellular counting 1 day and 7 days after mitomycin-C treatment was 232.5, 109.7 microg/ml and 239.9, 367.5 microg/ml, respectively. It is concluded that mitomycin-C is more toxic to cellular proliferation in cultured corneal keratocytes than in endothelial cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival / drug effects*
  • Cells, Cultured
  • Coloring Agents / pharmacokinetics
  • Cornea / drug effects*
  • Dose-Response Relationship, Drug
  • Endothelium, Corneal / drug effects*
  • In Vitro Techniques
  • Keratins / drug effects*
  • Mitomycin / toxicity*
  • Swine
  • Tetrazolium Salts / pharmacokinetics
  • Thiazoles / pharmacokinetics
  • Thymidine / metabolism
  • Time Factors

Substances

  • Coloring Agents
  • Tetrazolium Salts
  • Thiazoles
  • Mitomycin
  • Keratins
  • thiazolyl blue
  • Thymidine