Abstract
Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we demonstrate that the human homeodomain protein OTX2 is present in nuclear extracts of IRBP expressing cells and specifically interacts with the IRBP A promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very similar to OTX2, activates the human IRBP promoter in co-transfection experiments.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cattle
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Cell Line
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DNA / genetics*
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DNA / metabolism*
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DNA Primers / genetics
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Eye Proteins / genetics*
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HeLa Cells
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Humans
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In Vitro Techniques
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Otx Transcription Factors
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Photoreceptor Cells / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Retina / metabolism
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Retinol-Binding Proteins / genetics*
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Spodoptera
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transfection
Substances
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DNA Primers
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Eye Proteins
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Homeodomain Proteins
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Nerve Tissue Proteins
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OTX2 protein, human
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Otx Transcription Factors
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Recombinant Proteins
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Retinol-Binding Proteins
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Trans-Activators
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interstitial retinol-binding protein
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DNA