Abstract
Transforming growth factor-beta (TGF-beta) signaling is mediated by a complex of type I (TBRI) and type II (TBRII) receptors. The type III receptor (TBRIII) lacks a recognizable signaling domain and has no clearly defined role in TGF-beta signaling. Cardiac endothelial cells that undergo epithelial-mesenchymal transformation express TBRIII, and here TBRIII-specific antisera were found to inhibit mesenchyme formation and migration in atrioventricular cushion explants. Misexpression of TBRIII in nontransforming ventricular endothelial cells conferred transformation in response to TGF-beta2. These results support a model where TBRIII localizes transformation in the heart and plays an essential, nonredundant role in TGF-beta signaling.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Movement
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Chick Embryo
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Culture Techniques
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Endocardium / cytology
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Endocardium / embryology*
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Endocardium / metabolism
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Endothelium / cytology*
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Endothelium / embryology
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Endothelium / metabolism
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Genetic Vectors
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Heart / embryology*
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Heart Atria / cytology
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Heart Atria / embryology
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Heart Ventricles / cytology
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Heart Ventricles / embryology
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Heart Ventricles / virology
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Immune Sera
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Ligands
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Mesoderm / cytology*
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Mesoderm / metabolism
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Myocardium / cytology
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Myocardium / metabolism
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Protein Serine-Threonine Kinases
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Proteoglycans / immunology
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Proteoglycans / physiology*
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Receptor, Transforming Growth Factor-beta Type II
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Receptors, Transforming Growth Factor beta / immunology
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Receptors, Transforming Growth Factor beta / physiology*
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Retroviridae / genetics
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Retroviridae / physiology
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Signal Transduction
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Transforming Growth Factor beta / metabolism*
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Transforming Growth Factor beta / pharmacology
Substances
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Immune Sera
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Ligands
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Proteoglycans
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Receptors, Transforming Growth Factor beta
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Transforming Growth Factor beta
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betaglycan
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Protein Serine-Threonine Kinases
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Receptor, Transforming Growth Factor-beta Type II